Indlela yokuhlola ukwengezwa kwezinto ezingaphili i

Indlela yokuhlola ukwengezwa kwezinto ezingaphili ezinsizakalweni zokucubungula i-ACR:

Indlela yokuthola i-Ca2+:

Amathuluzi okuhlola kanye nama-reagent: i-beaker; Ibhodlela elibunjwe ngekhoni; I-Funnel; i-burette; Isithando sikagesi; I-ethanol engenamanzi; I-Hydrochloric acid, isixazululo se-buffer se-NH3-NH4Cl, inkomba ye-calcium, isixazululo esijwayelekile se-0.02mol/L EDTA.

Izinyathelo zokuhlola:

1. Kala ngokunembile inani elithile lesampula yosizo lokucubungula i-ACR (elinembile kufika ku-0.0001g) bese ulibeka ebhodleleni. Limanzise nge-ethanol engamanzi, bese ufaka i-hydrochloric acid engu-1:1 bese uyishisa esithandweni sikagesi ukuze uphendule ngokuphelele ama-ion e-calcium nge-hydrochloric acid;

2. Geza ngamanzi bese uhlunga nge-funnel ukuze uthole uketshezi olucacile;

3. Lungisa inani le-pH libe ngaphezu kuka-12 ngesisombululo se-buffer se-NH3-NH4Cl, engeza inani elifanele le-calcium indicator, bese ufaka i-titrate ngesisombululo esijwayelekile se-0.02mol/L EDTA. Isiphetho yilapho umbala ushintsha kusuka kubomvu onsomi uye kuluhlaza okwesibhakabhaka okumsulwa;

4. Yenza izivivinyo ezingenalutho ngesikhathi esisodwa;

5. Bala u-C # a2+=0.02 $(V-V0) $0.04004M $%&&

V – Umthamo (mL) wesisombululo se-EDTA esisetshenziswa lapho kuhlolwa amasampula osizo lokucubungula i-ACR.

V # – Umthamo wesisombululo esisetshenzisiwe ngesikhathi sokuhlolwa okungenalutho

M - Kala isisindo (g) sesampula yosizo lokucubungula i-ACR.

Indlela yokushisa yokulinganisa izinto ezingaphili:

Amathuluzi okuhlola: ibhalansi yokuhlaziya, isithando somlilo esithambile.

Izinyathelo zokuhlola: Thatha amasampula osizo lokucubungula i-ACR angu-0.5,1.0g (anembile ku-0.001g), uwabeke esithandweni sokushisa esingaguquki esingu-950 ihora eli-1, uphole, bese ukala ukuze ubale izinsalela zokusha ezisele. Uma izinto ezingaphili zengezwa kumasampula osizo lokucubungula i-ACR, kuzoba nezinsalela ezengeziwe.